Determinants of Product Outcome in Two Sesquiterpene Synthases from the Thermotolerant Bacterium Rubrobacter radiotolerans

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• TERPENOSOME: Engineered compartments for monoterpenoid production using synthetic biology

  Takano, E. (PI) & Scrutton, N. (CoI)

  1/07/14 → 30/06/17

  Project: Research

  • Synthase 100%
  • Escherichia coli 50%
  • Cyclization 38%
  • Cyclase 37%
  • Natural Product 33%

• Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

  Scrutton, N. (PI), Azapagic, A. (CoI), Balmer, A. (CoI), Barran, P. (CoI), Breitling, R. (CoI), Delneri, D. (CoI), Dixon, N. (CoI), Faulon, J.-L. (CoI), Flitsch, S. (CoI), Goble, C. (CoI), Goodacre, R. (CoI), Hay, S. (CoI), Kell, D. (CoI), Leys, D. (CoI), Lloyd, J. (CoI), Lockyer, N. (CoI), Martin, P. (CoI), Micklefield, J. (CoI), Munro, A. (CoI), Pedrosa Mendes, P. (CoI), Randles, S. (CoI), Salehi Yazdi, F. (CoI), Shapira, P. (CoI), Takano, E. (CoI), Turner, N. (CoI) & Winterburn, J. (CoI)

  14/11/14 → 13/05/20

  Project: Research

  • Metabolite 100%
  • Metabolic 99%
  • Oxidoreductase 92%
  • Escherichia coli 89%
  • Ion Mobility Mass Spectrometry 85%

• Innovative routes to monoterpene hydrocarbons and their high value derivatives

  Scrutton, N. (PI), Breitling, R. (CoI), Gardiner, J. (CoI), Hay, S. (CoI), Leys, D. (CoI), Pedrosa Mendes, P. (CoI) & Takano, E. (CoI)

  1/11/14 → 30/04/20

  Project: Research

  • Synthase 100%
  • Terpene 60%
  • Oxidoreductase 40%
  • Escherichia coli 40%
  • Cyclization 33%