Preanalytical variables and analytes in liquid biopsy approach for brain tumors: A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium

Chetan Bettegowda; Houtan Noushmehr; Alessandra Affinito; Manmeet S Ahluwalia; Olaf Ansorge; Katayoun Ayasoufi; Stephen Bagley; Jill Barnholtz-Sloan; Myron Best; Dieta Brandsma; Chaya Brodie; Anke Brüning-Richardson; Ana Valeria Castro; Susan M Chang; Gerolama Condorelli; Ahmad Daher; Vineet Datta; John de Groot; Pim French; Evanthia Galanis; Anna Golebiewska; Petra Hamerlik; C Oliver Hanemann; Matthias Holdhoff; Jason Huse; Mustafa Khasraw; Suzanne LeBlang; Beatrice Melin; Florent Mouliere; Claire O'Leary; Janusz Rak; Amitava Ray; Stephen Robinson; Ola Rominiyi; Federico Roncaroli; Roberta Rudà; Joan Seoane; Nik Sol; Martin J van den Bent; Michael A Vogelbaum; Tobias Walbert; Colin Watts; Tobias Weiss; Michael Weller; Patrick Y Wen; Victoria Wykes; Stephen Yip; Susan C Short; Riccardo Soffietti

doi:10.1093/neuonc/noaf140

Preanalytical variables and analytes in liquid biopsy approach for brain tumors: A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium

Chetan Bettegowda, Houtan Noushmehr, Alessandra Affinito, Manmeet S Ahluwalia, Olaf Ansorge, Katayoun Ayasoufi, Stephen Bagley, Jill Barnholtz-Sloan, Myron Best, Dieta Brandsma, Chaya Brodie, Anke Brüning-Richardson, Ana Valeria Castro, Susan M Chang, Gerolama Condorelli, Ahmad Daher, Vineet Datta, John de Groot, Pim French, Evanthia GalanisAnna Golebiewska, Petra Hamerlik, C Oliver Hanemann, Matthias Holdhoff, Jason Huse, Mustafa Khasraw, Suzanne LeBlang, Beatrice Melin, Florent Mouliere, Claire O'Leary, Janusz Rak, Amitava Ray, Stephen Robinson, Ola Rominiyi, Federico Roncaroli, Roberta Rudà, Joan Seoane, Nik Sol, Martin J van den Bent, Michael A Vogelbaum, Tobias Walbert, Colin Watts, Tobias Weiss, Michael Weller, Patrick Y Wen, Victoria Wykes, Stephen Yip, Susan C Short, Riccardo Soffietti

Research output: Contribution to journal › Article › peer-review

Abstract

This review explores the pivotal role of preanalytical variables in bringing liquid biopsy approaches into the clinic for brain tumors. Preanalytical variables encompass a range of critical issues, from blood sample collection and handling to the impact of tumor heterogeneity and patient-specific factors. These variables introduce challenges such as false positives, false negatives, and variability in the analysis of tumor signals, which can hinder the diagnostic and prognostic utility of liquid biopsies. Understanding the nuances of preanalytical variables is essential for the successful implementation of liquid biopsy in clinical settings. This paper delves into strategies aimed at mitigating the influence of preanalytical variables by emphasizing the importance of standardized sample collection protocols, optimized sample processing and storage, quality control measures, and the integration of multiple liquid biopsy modalities.

Original language	English
Journal	Neuro-Oncology
DOIs	https://doi.org/10.1093/neuonc/noaf140
Publication status	E-pub ahead of print - 30 Aug 2025

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10.1093/neuonc/noaf140

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Bettegowda, C., Noushmehr, H., Affinito, A., Ahluwalia, M. S., Ansorge, O., Ayasoufi, K., Bagley, S., Barnholtz-Sloan, J., Best, M., Brandsma, D., Brodie, C., Brüning-Richardson, A., Castro, A. V., Chang, S. M., Condorelli, G., Daher, A., Datta, V., de Groot, J., French, P., ... Soffietti, R. (2025). Preanalytical variables and analytes in liquid biopsy approach for brain tumors: A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium. Neuro-Oncology. Advance online publication. https://doi.org/10.1093/neuonc/noaf140

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title = "Preanalytical variables and analytes in liquid biopsy approach for brain tumors: A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium",

abstract = "This review explores the pivotal role of preanalytical variables in bringing liquid biopsy approaches into the clinic for brain tumors. Preanalytical variables encompass a range of critical issues, from blood sample collection and handling to the impact of tumor heterogeneity and patient-specific factors. These variables introduce challenges such as false positives, false negatives, and variability in the analysis of tumor signals, which can hinder the diagnostic and prognostic utility of liquid biopsies. Understanding the nuances of preanalytical variables is essential for the successful implementation of liquid biopsy in clinical settings. This paper delves into strategies aimed at mitigating the influence of preanalytical variables by emphasizing the importance of standardized sample collection protocols, optimized sample processing and storage, quality control measures, and the integration of multiple liquid biopsy modalities.",

author = "Chetan Bettegowda and Houtan Noushmehr and Alessandra Affinito and Ahluwalia, \{Manmeet S\} and Olaf Ansorge and Katayoun Ayasoufi and Stephen Bagley and Jill Barnholtz-Sloan and Myron Best and Dieta Brandsma and Chaya Brodie and Anke Br{\"u}ning-Richardson and Castro, \{Ana Valeria\} and Chang, \{Susan M\} and Gerolama Condorelli and Ahmad Daher and Vineet Datta and \{de Groot\}, John and Pim French and Evanthia Galanis and Anna Golebiewska and Petra Hamerlik and Hanemann, \{C Oliver\} and Matthias Holdhoff and Jason Huse and Mustafa Khasraw and Suzanne LeBlang and Beatrice Melin and Florent Mouliere and Claire O'Leary and Janusz Rak and Amitava Ray and Stephen Robinson and Ola Rominiyi and Federico Roncaroli and Roberta Rud{\`a} and Joan Seoane and Nik Sol and \{van den Bent\}, \{Martin J\} and Vogelbaum, \{Michael A\} and Tobias Walbert and Colin Watts and Tobias Weiss and Michael Weller and Wen, \{Patrick Y\} and Victoria Wykes and Stephen Yip and Short, \{Susan C\} and Riccardo Soffietti",

note = "{\textcopyright} The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For commercial re-use, please contact [email protected] for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact [email protected].",

year = "2025",

month = aug,

day = "30",

doi = "10.1093/neuonc/noaf140",

language = "English",

journal = "Neuro-Oncology",

issn = "1522-8517",

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Bettegowda, C, Noushmehr, H, Affinito, A, Ahluwalia, MS, Ansorge, O, Ayasoufi, K, Bagley, S, Barnholtz-Sloan, J, Best, M, Brandsma, D, Brodie, C, Brüning-Richardson, A, Castro, AV, Chang, SM, Condorelli, G, Daher, A, Datta, V, de Groot, J, French, P, Galanis, E, Golebiewska, A, Hamerlik, P, Hanemann, CO, Holdhoff, M, Huse, J, Khasraw, M, LeBlang, S, Melin, B, Mouliere, F, O'Leary, C, Rak, J, Ray, A, Robinson, S, Rominiyi, O, Roncaroli, F, Rudà, R, Seoane, J, Sol, N, van den Bent, MJ, Vogelbaum, MA, Walbert, T, Watts, C, Weiss, T, Weller, M, Wen, PY, Wykes, V, Yip, S, Short, SC & Soffietti, R 2025, 'Preanalytical variables and analytes in liquid biopsy approach for brain tumors: A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium', Neuro-Oncology. https://doi.org/10.1093/neuonc/noaf140

TY - JOUR

T1 - Preanalytical variables and analytes in liquid biopsy approach for brain tumors

T2 - A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium

AU - Bettegowda, Chetan

AU - Noushmehr, Houtan

AU - Affinito, Alessandra

AU - Ahluwalia, Manmeet S

AU - Ansorge, Olaf

AU - Ayasoufi, Katayoun

AU - Bagley, Stephen

AU - Barnholtz-Sloan, Jill

AU - Best, Myron

AU - Brandsma, Dieta

AU - Brodie, Chaya

AU - Brüning-Richardson, Anke

AU - Castro, Ana Valeria

AU - Chang, Susan M

AU - Condorelli, Gerolama

AU - Daher, Ahmad

AU - Datta, Vineet

AU - de Groot, John

AU - French, Pim

AU - Galanis, Evanthia

AU - Golebiewska, Anna

AU - Hamerlik, Petra

AU - Hanemann, C Oliver

AU - Holdhoff, Matthias

AU - Huse, Jason

AU - Khasraw, Mustafa

AU - LeBlang, Suzanne

AU - Melin, Beatrice

AU - Mouliere, Florent

AU - O'Leary, Claire

AU - Rak, Janusz

AU - Ray, Amitava

AU - Robinson, Stephen

AU - Rominiyi, Ola

AU - Roncaroli, Federico

AU - Rudà, Roberta

AU - Seoane, Joan

AU - Sol, Nik

AU - van den Bent, Martin J

AU - Vogelbaum, Michael A

AU - Walbert, Tobias

AU - Watts, Colin

AU - Weiss, Tobias

AU - Weller, Michael

AU - Wen, Patrick Y

AU - Wykes, Victoria

AU - Yip, Stephen

AU - Short, Susan C

AU - Soffietti, Riccardo

N1 - © The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For commercial re-use, please contact [email protected] for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact [email protected].

PY - 2025/8/30

Y1 - 2025/8/30

N2 - This review explores the pivotal role of preanalytical variables in bringing liquid biopsy approaches into the clinic for brain tumors. Preanalytical variables encompass a range of critical issues, from blood sample collection and handling to the impact of tumor heterogeneity and patient-specific factors. These variables introduce challenges such as false positives, false negatives, and variability in the analysis of tumor signals, which can hinder the diagnostic and prognostic utility of liquid biopsies. Understanding the nuances of preanalytical variables is essential for the successful implementation of liquid biopsy in clinical settings. This paper delves into strategies aimed at mitigating the influence of preanalytical variables by emphasizing the importance of standardized sample collection protocols, optimized sample processing and storage, quality control measures, and the integration of multiple liquid biopsy modalities.

AB - This review explores the pivotal role of preanalytical variables in bringing liquid biopsy approaches into the clinic for brain tumors. Preanalytical variables encompass a range of critical issues, from blood sample collection and handling to the impact of tumor heterogeneity and patient-specific factors. These variables introduce challenges such as false positives, false negatives, and variability in the analysis of tumor signals, which can hinder the diagnostic and prognostic utility of liquid biopsies. Understanding the nuances of preanalytical variables is essential for the successful implementation of liquid biopsy in clinical settings. This paper delves into strategies aimed at mitigating the influence of preanalytical variables by emphasizing the importance of standardized sample collection protocols, optimized sample processing and storage, quality control measures, and the integration of multiple liquid biopsy modalities.

U2 - 10.1093/neuonc/noaf140

DO - 10.1093/neuonc/noaf140

M3 - Article

C2 - 40884415

SN - 1522-8517

JO - Neuro-Oncology

JF - Neuro-Oncology

ER -

Preanalytical variables and analytes in liquid biopsy approach for brain tumors: A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium

Abstract

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