Proteomic analysis of integrin-associated complexes from mesenchymal stem cells

Jila N. Ajeian; Edward Horton; Pablo Astudillo; Adam Byron; Janet Askari; Angelique Millon Fremillon; David Knight; Susan Kimber; Martin J Humphries; Jonathan D Humphries

doi:10.1002/prca.201500033

Proteomic analysis of integrin-associated complexes from mesenchymal stem cells

Jila N. Ajeian, Edward Horton, Pablo Astudillo, Adam Byron, Janet Askari, Angelique Millon Fremillon, David Knight, Susan Kimber, Martin J Humphries, Jonathan D Humphries

Division of Cell Matrix Biology & Regenerative Medicine (L5)

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Abstract

Multipotent mesenchymal stem cells (MSCs) have the capability to differentiate down adipocyte, osteocyte and chondrocyte lineages and as such offer a range of potential therapeutic applications. The composition and stiffness of the extracellular matrix (ECM) environment that surrounds cells dictates their transcriptional programme, thereby affecting stem cell lineage decision-making. Cells sense force via linkages between themselves and their microenvironment, and this is transmitted by integrin receptors and associated adhesion signalling complexes. To identify regulators of MSC force sensing, we sought to catalogue MSC integrin-associated adhesion complex composition.

Original language	English
Pages (from-to)	51-57
Number of pages	6
Journal	Proteomics - Clinical Applications
Volume	10
Issue number	1
Early online date	6 Jul 2015
DOIs	https://doi.org/10.1002/prca.201500033
Publication status	Published - 5 Jan 2016

Keywords

Extracellular matrix
Integrin
LIM domain
Mechanotransduction
Mesenchymal stem cell

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10.1002/prca.201500033Licence: CC BY

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Biological Mass Spectrometry (BioMS) Facility
Knight, D. (Core Facility Lead), Warwood, S. (Senior Technical Specialist), Selley, J. (Technical Specialist), Taylor, G. (Technical Specialist), Fullwood, P. (Technical Specialist), Keevill, E.-J. (Senior Technician) & Allsey, J. (Technician)
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Cite this

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title = "Proteomic analysis of integrin-associated complexes from mesenchymal stem cells",

abstract = "Multipotent mesenchymal stem cells (MSCs) have the capability to differentiate down adipocyte, osteocyte and chondrocyte lineages and as such offer a range of potential therapeutic applications. The composition and stiffness of the extracellular matrix (ECM) environment that surrounds cells dictates their transcriptional programme, thereby affecting stem cell lineage decision-making. Cells sense force via linkages between themselves and their microenvironment, and this is transmitted by integrin receptors and associated adhesion signalling complexes. To identify regulators of MSC force sensing, we sought to catalogue MSC integrin-associated adhesion complex composition.",

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AU - Ajeian, Jila N.

AU - Horton, Edward

AU - Astudillo, Pablo

AU - Byron, Adam

AU - Askari, Janet

AU - Millon Fremillon, Angelique

AU - Knight, David

AU - Kimber, Susan

AU - Humphries, Martin J

AU - Humphries, Jonathan D

PY - 2016/1/5

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N2 - Multipotent mesenchymal stem cells (MSCs) have the capability to differentiate down adipocyte, osteocyte and chondrocyte lineages and as such offer a range of potential therapeutic applications. The composition and stiffness of the extracellular matrix (ECM) environment that surrounds cells dictates their transcriptional programme, thereby affecting stem cell lineage decision-making. Cells sense force via linkages between themselves and their microenvironment, and this is transmitted by integrin receptors and associated adhesion signalling complexes. To identify regulators of MSC force sensing, we sought to catalogue MSC integrin-associated adhesion complex composition.

AB - Multipotent mesenchymal stem cells (MSCs) have the capability to differentiate down adipocyte, osteocyte and chondrocyte lineages and as such offer a range of potential therapeutic applications. The composition and stiffness of the extracellular matrix (ECM) environment that surrounds cells dictates their transcriptional programme, thereby affecting stem cell lineage decision-making. Cells sense force via linkages between themselves and their microenvironment, and this is transmitted by integrin receptors and associated adhesion signalling complexes. To identify regulators of MSC force sensing, we sought to catalogue MSC integrin-associated adhesion complex composition.

KW - Extracellular matrix

KW - Integrin

KW - LIM domain

KW - Mechanotransduction

KW - Mesenchymal stem cell

U2 - 10.1002/prca.201500033

DO - 10.1002/prca.201500033

M3 - Article

SN - 1862-8346

VL - 10

SP - 51

EP - 57

JO - Proteomics - Clinical Applications

JF - Proteomics - Clinical Applications

IS - 1

ER -

Proteomic analysis of integrin-associated complexes from mesenchymal stem cells

Abstract

Keywords

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