Protocol to characterize basement membranes during kidney development using mass spectrometry-based label-free quantitative proteomics

Mychel R.P.T. Morais; Pinyuan Tian; Ronan O'cualain; Craig Lawless; Rachel Lennon

doi:10.1016/j.xpro.2023.102741

Protocol to characterize basement membranes during kidney development using mass spectrometry-based label-free quantitative proteomics

Mychel R.P.T. Morais, Pinyuan Tian^*, Ronan O'cualain, Craig Lawless, Rachel Lennon^*

^*Corresponding author for this work

Wellcome Centre for Cell‐Matrix Research, The University of Manchester

Research output: Contribution to journal › Article › peer-review

Abstract

Basement membranes are specialized extracellular matrices formed by highly insoluble structural proteins and extracellular matrix (ECM)-bound components that provide structural and signaling support to tissues and are dynamic during development. Here, we present a mass spectrometry-based label-free quantitative proteomics protocol to investigate basement membranes and define their composition using samples from human kidney organoids and mouse fetal kidneys. This protocol facilitates the study of basement membrane and other ECM components during development to improve our understanding of matrix regulation and function. For complete details on the use and execution of this protocol, please refer to Morais et al.¹

Original language	English
Article number	102741
Journal	STAR protocols
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2023.102741
Publication status	Published - 15 Dec 2023

Keywords

Mass Spectrometry
Metabolism
Proteomics

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10.1016/j.xpro.2023.102741Licence: CC BY

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title = "Protocol to characterize basement membranes during kidney development using mass spectrometry-based label-free quantitative proteomics",

abstract = "Basement membranes are specialized extracellular matrices formed by highly insoluble structural proteins and extracellular matrix (ECM)-bound components that provide structural and signaling support to tissues and are dynamic during development. Here, we present a mass spectrometry-based label-free quantitative proteomics protocol to investigate basement membranes and define their composition using samples from human kidney organoids and mouse fetal kidneys. This protocol facilitates the study of basement membrane and other ECM components during development to improve our understanding of matrix regulation and function. For complete details on the use and execution of this protocol, please refer to Morais et al.1",

keywords = "Mass Spectrometry, Metabolism, Proteomics",

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AU - Morais, Mychel R.P.T.

AU - Tian, Pinyuan

AU - O'cualain, Ronan

AU - Lawless, Craig

AU - Lennon, Rachel

PY - 2023/12/15

Y1 - 2023/12/15

N2 - Basement membranes are specialized extracellular matrices formed by highly insoluble structural proteins and extracellular matrix (ECM)-bound components that provide structural and signaling support to tissues and are dynamic during development. Here, we present a mass spectrometry-based label-free quantitative proteomics protocol to investigate basement membranes and define their composition using samples from human kidney organoids and mouse fetal kidneys. This protocol facilitates the study of basement membrane and other ECM components during development to improve our understanding of matrix regulation and function. For complete details on the use and execution of this protocol, please refer to Morais et al.1

AB - Basement membranes are specialized extracellular matrices formed by highly insoluble structural proteins and extracellular matrix (ECM)-bound components that provide structural and signaling support to tissues and are dynamic during development. Here, we present a mass spectrometry-based label-free quantitative proteomics protocol to investigate basement membranes and define their composition using samples from human kidney organoids and mouse fetal kidneys. This protocol facilitates the study of basement membrane and other ECM components during development to improve our understanding of matrix regulation and function. For complete details on the use and execution of this protocol, please refer to Morais et al.1

KW - Mass Spectrometry

KW - Metabolism

KW - Proteomics

UR - https://www.scopus.com/pages/publications/85182210601

U2 - 10.1016/j.xpro.2023.102741

DO - 10.1016/j.xpro.2023.102741

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AN - SCOPUS:85182210601

SN - 2666-1667

VL - 4

JO - STAR protocols

JF - STAR protocols

IS - 4

M1 - 102741

ER -

Protocol to characterize basement membranes during kidney development using mass spectrometry-based label-free quantitative proteomics

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Biological Mass Spectrometry (BioMS) Facility

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Protocol to characterize basement membranes during kidney development using mass spectrometry-based label-free quantitative proteomics

Abstract

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Biological Mass Spectrometry (BioMS) Facility

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