The p75 neurotrophin receptor appears in plasma in diabetic rats - Characterisation of a potential early test for neuropathy

L. Chilton; A. Middlemas; N. Gardiner; D. R. Tomlinson

doi:10.1007/s00125-004-1550-0

The p75 neurotrophin receptor appears in plasma in diabetic rats - Characterisation of a potential early test for neuropathy

L. Chilton, A. Middlemas, N. Gardiner, D. R. Tomlinson

Research output: Contribution to journal › Article › peer-review

Abstract

Aims/hypothesis. This study tested the premise that immunoreactivity representing the p75 neurotrophin receptor (p75NTR) appears in plasma of diabetic rats in association with the early stages of neuronal dysfunction or damage. We also examined whether treatment beneficial to neuropathy might reduce the p75NTR immunoreactivity. Methods. Plasma proteins were fractionated by SDS-PAGE and immunoblots exposed to p75NTR antibody, using receptor protein from cultured PC12 cells as an external standard. Rats were made diabetic with streptozotocin for various periods and exsanguinated. Plasma glucose, HbA1C and plasma proteins were determined. We also studied plasma samples from diabetic mice lacking the gene coding for p75 NTR, as well as the effect of sciatic nerve crush on healthy male Wistar rats. Results. Plasma p75NTR immunoreactivity began to exceed normal levels at 8 weeks after induction of diabates, and was significantly raised at 10 (p

Original language	English
Pages (from-to)	1924-1930
Number of pages	6
Journal	Diabetologia
Volume	47
Issue number	11
DOIs	https://doi.org/10.1007/s00125-004-1550-0
Publication status	Published - Nov 2004

Keywords

Nerve growth factor
Neuropathy
Neurotrophins
p75NTR
Plasma marker
Rats
Receptor shedding

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00125-004-1550-0

Cite this

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title = "The p75 neurotrophin receptor appears in plasma in diabetic rats - Characterisation of a potential early test for neuropathy",

abstract = "Aims/hypothesis. This study tested the premise that immunoreactivity representing the p75 neurotrophin receptor (p75NTR) appears in plasma of diabetic rats in association with the early stages of neuronal dysfunction or damage. We also examined whether treatment beneficial to neuropathy might reduce the p75NTR immunoreactivity. Methods. Plasma proteins were fractionated by SDS-PAGE and immunoblots exposed to p75NTR antibody, using receptor protein from cultured PC12 cells as an external standard. Rats were made diabetic with streptozotocin for various periods and exsanguinated. Plasma glucose, HbA1C and plasma proteins were determined. We also studied plasma samples from diabetic mice lacking the gene coding for p75 NTR, as well as the effect of sciatic nerve crush on healthy male Wistar rats. Results. Plasma p75NTR immunoreactivity began to exceed normal levels at 8 weeks after induction of diabates, and was significantly raised at 10 (p",

keywords = "Nerve growth factor, Neuropathy, Neurotrophins, p75NTR, Plasma marker, Rats, Receptor shedding",

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doi = "10.1007/s00125-004-1550-0",

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T1 - The p75 neurotrophin receptor appears in plasma in diabetic rats - Characterisation of a potential early test for neuropathy

AU - Chilton, L.

AU - Middlemas, A.

AU - Gardiner, N.

AU - Tomlinson, D. R.

PY - 2004/11

Y1 - 2004/11

N2 - Aims/hypothesis. This study tested the premise that immunoreactivity representing the p75 neurotrophin receptor (p75NTR) appears in plasma of diabetic rats in association with the early stages of neuronal dysfunction or damage. We also examined whether treatment beneficial to neuropathy might reduce the p75NTR immunoreactivity. Methods. Plasma proteins were fractionated by SDS-PAGE and immunoblots exposed to p75NTR antibody, using receptor protein from cultured PC12 cells as an external standard. Rats were made diabetic with streptozotocin for various periods and exsanguinated. Plasma glucose, HbA1C and plasma proteins were determined. We also studied plasma samples from diabetic mice lacking the gene coding for p75 NTR, as well as the effect of sciatic nerve crush on healthy male Wistar rats. Results. Plasma p75NTR immunoreactivity began to exceed normal levels at 8 weeks after induction of diabates, and was significantly raised at 10 (p

AB - Aims/hypothesis. This study tested the premise that immunoreactivity representing the p75 neurotrophin receptor (p75NTR) appears in plasma of diabetic rats in association with the early stages of neuronal dysfunction or damage. We also examined whether treatment beneficial to neuropathy might reduce the p75NTR immunoreactivity. Methods. Plasma proteins were fractionated by SDS-PAGE and immunoblots exposed to p75NTR antibody, using receptor protein from cultured PC12 cells as an external standard. Rats were made diabetic with streptozotocin for various periods and exsanguinated. Plasma glucose, HbA1C and plasma proteins were determined. We also studied plasma samples from diabetic mice lacking the gene coding for p75 NTR, as well as the effect of sciatic nerve crush on healthy male Wistar rats. Results. Plasma p75NTR immunoreactivity began to exceed normal levels at 8 weeks after induction of diabates, and was significantly raised at 10 (p

KW - Nerve growth factor

KW - Neuropathy

KW - Neurotrophins

KW - p75NTR

KW - Plasma marker

KW - Rats

KW - Receptor shedding

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SN - 1432-0428

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EP - 1930

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The p75 neurotrophin receptor appears in plasma in diabetic rats - Characterisation of a potential early test for neuropathy

Abstract

Keywords

UN SDGs

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