Abstract
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 277-281 |
| Number of pages | 4 |
| Journal | Surface and Interface Analysis |
| Volume | 45 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2013 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- cancer
- doxorubicin
- hypoxia
- metabolomics
- PCA
- ToF-SIMS
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology
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