Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

BreathDx Consortium; Pouline Mp van Oort; Tamara M Nijsen; Iain R White; Hugo H Knobel; Timothy Felton; Nicholas Rattray; Oluwasola Lawal; Murtaza Bulut; Waqar Ahmed; Antonio Artigas; Pedro R Povoa; Ignacio Martin-Loeches; Hans Weda; Royston Goodacre; Marcus J Schultz; Paul M Dark; Stephen J Fowler; Lieuwe D Bos

doi:10.1136/thoraxjnl-2021-217362

Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

BreathDx Consortium
, Pouline Mp van Oort
, Tamara M Nijsen
, Iain R White
, Hugo H Knobel
, Timothy Felton
, Nicholas Rattray
, Oluwasola Lawal
, Murtaza Bulut
, Waqar Ahmed
, Antonio Artigas
, Pedro R Povoa
, Ignacio Martin-Loeches
, Hans Weda
, Royston Goodacre
, Marcus J Schultz
, Paul M Dark
, Stephen J Fowler
, Lieuwe D Bos

Division of Immunology, Immunity to Infection and Respiratory Medicine

Philips Research Laboratories
Centro Hospitalar Lisboa Central EPE
University of Dublin Trinity College
Orikami
Eurofins Materials Science Netherlands BV
Hospital Parc Taulí
University of Liverpool
Amsterdam University Medical Centre (Amsterdam UMC)

Research output: Contribution to journal › Article › peer-review

Abstract

Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

Original language	English
Pages (from-to)	79-81
Number of pages	3
Journal	Thorax
Volume	77
Issue number	1
Early online date	4 Jul 2021
DOIs	https://doi.org/10.1136/thoraxjnl-2021-217362
Publication status	Published - Jan 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

assisted ventilation
critical care
pneumonia

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

Access to Document

10.1136/thoraxjnl-2021-217362

Cite this

BreathDx Consortium, van Oort, P. M., Nijsen, T. M., White, I. R., Knobel, H. H., Felton, T., Rattray, N., Lawal, O., Bulut, M., Ahmed, W., Artigas, A., Povoa, P. R., Martin-Loeches, I., Weda, H., Goodacre, R., Schultz, M. J., Dark, P. M., Fowler, S. J., & Bos, L. D. (2022). Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx). Thorax, 77(1), 79-81. https://doi.org/10.1136/thoraxjnl-2021-217362

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title = "Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)",

abstract = "Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98\% at a specificity of 49\%, confirmed with a second analytical method. The breath test had a negative predictive value of 96\% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.",

keywords = "assisted ventilation, critical care, pneumonia",

author = "\{BreathDx Consortium\} and \{van Oort\}, \{Pouline Mp\} and Nijsen, \{Tamara M\} and White, \{Iain R\} and Knobel, \{Hugo H\} and Timothy Felton and Nicholas Rattray and Oluwasola Lawal and Murtaza Bulut and Waqar Ahmed and Antonio Artigas and Povoa, \{Pedro R\} and Ignacio Martin-Loeches and Hans Weda and Royston Goodacre and Schultz, \{Marcus J\} and Dark, \{Paul M\} and Fowler, \{Stephen J\} and Bos, \{Lieuwe D\}",

note = "Funding Information: Funding The project was funded by the European Union FP7 Marie Curie Actions, under the Industry-Academia Partnerships and Pathways (IAPP) programme (MC-IAPP BreathDx 611951). IRW, TF, PMD and SJF were supported by the NIHR Manchester Biochemical Research Centre. Competing interests None declared. Patient consent for publication Not required. Publisher Copyright: {\textcopyright}",

year = "2022",

month = jan,

doi = "10.1136/thoraxjnl-2021-217362",

language = "English",

volume = "77",

pages = "79--81",

journal = "Thorax",

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BreathDx Consortium, van Oort, PM, Nijsen, TM, White, IR, Knobel, HH, Felton, T, Rattray, N, Lawal, O, Bulut, M, Ahmed, W, Artigas, A, Povoa, PR, Martin-Loeches, I, Weda, H, Goodacre, R, Schultz, MJ, Dark, PM , Fowler, SJ & Bos, LD 2022, 'Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)', Thorax, vol. 77, no. 1, pp. 79-81. https://doi.org/10.1136/thoraxjnl-2021-217362

TY - JOUR

T1 - Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

AU - BreathDx Consortium

AU - van Oort, Pouline Mp

AU - Nijsen, Tamara M

AU - White, Iain R

AU - Knobel, Hugo H

AU - Felton, Timothy

AU - Rattray, Nicholas

AU - Lawal, Oluwasola

AU - Bulut, Murtaza

AU - Ahmed, Waqar

AU - Artigas, Antonio

AU - Povoa, Pedro R

AU - Martin-Loeches, Ignacio

AU - Weda, Hans

AU - Goodacre, Royston

AU - Schultz, Marcus J

AU - Dark, Paul M

AU - Fowler, Stephen J

AU - Bos, Lieuwe D

N1 - Funding Information: Funding The project was funded by the European Union FP7 Marie Curie Actions, under the Industry-Academia Partnerships and Pathways (IAPP) programme (MC-IAPP BreathDx 611951). IRW, TF, PMD and SJF were supported by the NIHR Manchester Biochemical Research Centre. Competing interests None declared. Patient consent for publication Not required. Publisher Copyright: ©

PY - 2022/1

Y1 - 2022/1

N2 - Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

AB - Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

KW - assisted ventilation

KW - critical care

KW - pneumonia

U2 - 10.1136/thoraxjnl-2021-217362

DO - 10.1136/thoraxjnl-2021-217362

M3 - Article

C2 - 34088787

SN - 0040-6376

VL - 77

SP - 79

EP - 81

JO - Thorax

JF - Thorax

IS - 1

ER -

Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

Abstract

UN SDGs

Keywords

Research Beacons, Institutes and Platforms

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