Visualisation of plastid degradation in sperm cells of wheat pollen

Lucia F Primavesi; Huixia Wu; Elisabeth A Mudd; Anil Day; Huw D Jones

doi:10.1007/s00709-015-0935-x

Visualisation of plastid degradation in sperm cells of wheat pollen

Lucia F Primavesi, Huixia Wu, Elisabeth A Mudd, Anil Day, Huw D Jones

Research output: Contribution to journal › Article › peer-review

Abstract

Like most angiosperms, wheat (Triticum aestivum) shows maternal inheritance of plastids. It is thought that this takes place by cytoplasmic stripping at fertilisation rather than the absence of plastids in sperm cells. To determine the fate of plastids during sperm cell development, plastid-targeted green fluorescent protein was used to visualise these organelles in nuclear transgenic wheat lines. Fewer than thirty small 1–2-μm plastids were visible in early uninucleate pollen cells. These dramatically increased to several hundred larger (4 μm) plastids during pollen maturation and went through distinct morphological changes. Only small plastids were visible in generative cells (n = 25) and young sperm cells (n = 9). In mature sperm cells, these green fluorescent protein (GFP)-tagged plastids were absent. This is consistent with maternal inheritance of plastids resulting from their degradation in mature sperm cells in wheat.

Original language	English
Journal	Protoplasma
DOIs	https://doi.org/10.1007/s00709-015-0935-x
Publication status	Published - 21 Jan 2016

Keywords

Wheat – Pollen – GFP – Plastid – Sperm cell – Plastid networks – Maternal inheritance

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10.1007/s00709-015-0935-xLicence: Other

http://www.bbsrc.ac.uk/news/fundamental-bioscience/2016/160126-pr-unravelling-chloroplast-inheritance-in-wheat/Licence: Other

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title = "Visualisation of plastid degradation in sperm cells of wheat pollen",

abstract = "Like most angiosperms, wheat (Triticum aestivum) shows maternal inheritance of plastids. It is thought that this takes place by cytoplasmic stripping at fertilisation rather than the absence of plastids in sperm cells. To determine the fate of plastids during sperm cell development, plastid-targeted green fluorescent protein was used to visualise these organelles in nuclear transgenic wheat lines. Fewer than thirty small 1–2-μm plastids were visible in early uninucleate pollen cells. These dramatically increased to several hundred larger (4 μm) plastids during pollen maturation and went through distinct morphological changes. Only small plastids were visible in generative cells (n = 25) and young sperm cells (n = 9). In mature sperm cells, these green fluorescent protein (GFP)-tagged plastids were absent. This is consistent with maternal inheritance of plastids resulting from their degradation in mature sperm cells in wheat.",

keywords = "Wheat – Pollen – GFP – Plastid – Sperm cell – Plastid networks – Maternal inheritance",

author = "Primavesi, {Lucia F} and Huixia Wu and Mudd, {Elisabeth A} and Anil Day and Jones, {Huw D}",

year = "2016",

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doi = "10.1007/s00709-015-0935-x",

language = "English",

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T1 - Visualisation of plastid degradation in sperm cells of wheat pollen

AU - Primavesi, Lucia F

AU - Wu, Huixia

AU - Mudd, Elisabeth A

AU - Day, Anil

AU - Jones, Huw D

PY - 2016/1/21

Y1 - 2016/1/21

N2 - Like most angiosperms, wheat (Triticum aestivum) shows maternal inheritance of plastids. It is thought that this takes place by cytoplasmic stripping at fertilisation rather than the absence of plastids in sperm cells. To determine the fate of plastids during sperm cell development, plastid-targeted green fluorescent protein was used to visualise these organelles in nuclear transgenic wheat lines. Fewer than thirty small 1–2-μm plastids were visible in early uninucleate pollen cells. These dramatically increased to several hundred larger (4 μm) plastids during pollen maturation and went through distinct morphological changes. Only small plastids were visible in generative cells (n = 25) and young sperm cells (n = 9). In mature sperm cells, these green fluorescent protein (GFP)-tagged plastids were absent. This is consistent with maternal inheritance of plastids resulting from their degradation in mature sperm cells in wheat.

AB - Like most angiosperms, wheat (Triticum aestivum) shows maternal inheritance of plastids. It is thought that this takes place by cytoplasmic stripping at fertilisation rather than the absence of plastids in sperm cells. To determine the fate of plastids during sperm cell development, plastid-targeted green fluorescent protein was used to visualise these organelles in nuclear transgenic wheat lines. Fewer than thirty small 1–2-μm plastids were visible in early uninucleate pollen cells. These dramatically increased to several hundred larger (4 μm) plastids during pollen maturation and went through distinct morphological changes. Only small plastids were visible in generative cells (n = 25) and young sperm cells (n = 9). In mature sperm cells, these green fluorescent protein (GFP)-tagged plastids were absent. This is consistent with maternal inheritance of plastids resulting from their degradation in mature sperm cells in wheat.

KW - Wheat – Pollen – GFP – Plastid – Sperm cell – Plastid networks – Maternal inheritance

U2 - 10.1007/s00709-015-0935-x

DO - 10.1007/s00709-015-0935-x

M3 - Article

JO - Protoplasma

JF - Protoplasma

ER -

Visualisation of plastid degradation in sperm cells of wheat pollen

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